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UPDATE: The National Weather Service says Lincoln is experiencing an EF1 tornado.
A day after a tornado touched down in rural Lincoln, Nebraska, damage to parts of the city increased, though the number of confirmed injuries have decreased compared to earlier estimates.
Up to five homes had been damaged in the city, including the home of the mayor, Eric Braestrup, during the Friday tornado, according to meteorologist Rachel Volkert of the National Weather Service.
Five homes have been damaged, according to the Lincoln Police Department.
25.86726 -100.133286Molecular cloning and functional expression of voltage-dependent K+ channel in guinea pig esophagus.
To investigate the possible involvement of voltage-dependent K+ channels in esophageal mucosal protection, guinea pig esophageal epithelial cells were isolated and maintained in primary culture, and poly(A+) RNA was extracted. Poly(A+) RNA from guinea pig esophageal epithelial cells was used as template for a PCR reaction using a pair of degenerate primers with consensus nucleotide sequences of the nucleotide-binding domains (NBDs) of voltage-dependent K+ channels (Kv). The amplified products were partially sequenced and found to have 90-95% similarity to Kv1.1 and Kv1.2. The full-length cDNA of Kv7.1 was obtained from guinea pig esophageal epithelial cell cDNA library, which was designated as GPKv7.1. The open reading frame of GPKv7.1 was 2290 bp, which encoded a 751-amino-acid peptide with five putative transmembrane 0b46394aab