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Listeria monocytogenes is a foodborne pathogen that causes listeriosis, an important disease that can have fatal consequences for pregnant women, newborns and the immuno-compromised. Despite the low infectious dose of L. monocytogenes, molecular methods that target this pathogen are still available but only to a limited number of laboratories. Among them, most studies have emphasized the development of molecular methods targeting the listerial chromosome in which the virulence and the accessory genes are encoded. In this study, we developed a TaqMan-based quantitative PCR (qPCR) with a highly specific MGB probe to detect the real-time expression of hlyA gene in L. monocytogenes as a tool for L. monocytogenes detection in food. This method was validated using suspensions of L. monocytogenes serotypes 1/2a, 1/2b, 4b and 4b+4c, inoculated in meat (raw beef, chicken, pork and lamb). L. monocytogenes was detected within two hours of enrichment at a temperature of 35°C. The real-time 0b46394aab